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ADCP: When using the R/V Moana Wave (or equivalently equipped research vessel), we record upper water column currents using a hull- mounted acoustic doppler current profiler (ADCP). Additional details on ADCP- based ocean current measurements are presented elsewhere (see Chapter 3. Sampling: Discrete Depth Measurements. The HOT program sampling strategy is based, to the extent possible, on resolving depth profiles of specific chemical constituents from contiguous casts. This is done in order to produce profiles that minimize the effects of time- dependent density structure variation within the water column. Sampling depths range from the surface to approximately 5- 1. Approximately 2. 0% of the samples are collected and analyzed in triplicate. To the extent possible, samples are collected from the same depths and/or density levels each month to facilitate comparisons between monthly profiles. Salinity samples are collected from each water bottle in order to identify trip errors. JGOFS- related chemical sampling is concentrated in the upper 1. WOCE hydrographic sampling is conducted from the surface to approximately 5- 1. The WOCE program collects samples for inorganic nutrients, oxygen and salinity at 2. Flux Measurements. The rate of primary production is measured using 1. C trace- metal clean methods. Incubations are conducted either on deck or in situ (see Chapter 2. From HOT- 1 (Oct 1. HOT- 7 (June 1. 98. Between HOT- 8 (July 1. HOT- 1. 7 (May 1. Since HOT- 1. 8 (June 1. Particle fluxes are measured using both free- drifting sediment traps (see Chapter 2. Chapter 2. 9). 3. References. Chiswell, S. Intra- annual oscillations at Station ALOHA, north of Oahu, Hawaii. Deep- Sea Research II, 4. Chapter 5. QUALITY ASSURANCE / QUALITY CONTROL PROGRAMS. In order to provide accurate and reliable data to the oceanographic community, the JGOFS component of HOT has established a quality assurance / quality control (QA/QC) program that is designed to assess and maintain data quality. These QA/QC procedures encompass all aspects of the program from sample collection through data reporting. Introduction. The QA/QC programs are designed to ensure that data of the highest quality are obtained from the HOT sampling and measurement programs. A fundamental component of this QA/QC program is the documentation of the detailed analytical procedures that are presented in the following chapters of this manual. These procedures are consistently applied in our laboratory analyses. This chapter describes the QA/QC procedures that are independent of the specific analytical protocols presented in subsequent chapters. These QA/QC procedures include field sampling, analytical facilities and instrument maintenance, interlaboratory comparisons and data reporting. Precision and Accuracy. The precision and accuracy of each analytical procedure is discussed in the appropriate chapters. Accuracy is a measure of how close an analyzed value is to the true value. In general, the accuracy of an analytical method is determined by the use of calibrated, traceable reference standards. However, it is important to bear in mind that the assessment of accuracy based upon primary standards can be misleading if the standards are not prepared in seawater because many of our chemical determinations exhibit matrix (e. In addition, it must also be recognized that most of the HOT program core measurement parameters (e. H, primary production, particle flux, etc.), do not have readily available reference materials. Precision is a measure of the variability of individual measurements (i. In the HOT program, two categories of replicates are measured: field and analytical. Analytical replication is determined by repeated analysis of a single sample and is a measure of the greatest precision possible for a particular analysis. Field replication is determined by the analysis of two or more samples taken from a single sampling bottle and has an added component of variance due to subsampling, storage and natural between sample variability. The variance of field and analytical replicates should be equal when sampling and storage have no effect on the analysis (assuming the analyte is homogenously distributed within the sampling bottle). Therefore, the difference between field and analytical replicates provides a first order evaluation of the field sampling procedure. Higher level variance due to sample bottle replication (multiple bottles on same cast or multiple casts) is not well- resolved in the current HOT sampling procedures. It is apparent from these definitions that precision and accuracy are not necessarily coupled. An analysis may be precise yet inaccurate, whereas the mean of a variable result may be quite accurate. Therefore, precision and accuracy must be evaluated independently. Overview of the Quality Assurance /Quality Control Program. The basic framework of the HOT- JGOFS QA/QC program addresses field sampling, laboratory facilities, laboratory analysis and data reporting. Quality control in the field is primarily attained by utilizing modern sampling equipment that is properly maintained. The instruments receive periodic maintenance and calibration. Laboratory analyses are periodically reviewed for stepwise methodology, variance evaluations (control charts), reference materials (where available) and inter- and intralaboratory comparisons. Quality control procedures associated with data reporting include sample documentation, tracking and evaluation of analytical results, relative to sample documentation, and comparison of results to historical values. Field Sampling Strategy. Specific aspects of the time- series field sampling strategy have been presented elsewhere (see Chapter 2), and will not be repeated here except to emphasize key aspects of our QA/QC program. KAHE (see Figure 1. KAHE, an initial cast is performed using only a weight to test the winch and to inspect the condition of the hydrowire. This test cast is followed by a CTD- rosette cast to 1. The latter serves to test the CTD, pylon and deck box, to collect water column samples and to provide a hands- on opportunity for novice members of the scientific party to participate in the deployment and retrieval of the rosette and the collection of water samples prior to the more extensive collections at Sta. Ideally, samples are collected and processed exclusively by experienced personnel. However, the HOT program scientists encourage graduate and undergraduate student participation and endeavor to combine marine research with marine science education. Consequently, we conscientiously schedule an at- sea training session to ensure that the procedures followed are consistent from month- to- month. During each HOT cruise, at least 2. In addition, salinity samples are drawn and on- deck sample temperatures (for those casts where O2 samples are drawn) recorded from each water bottle sampled. Both procedures are useful for the identification of sample trip errors (i. The collection of representative samples is paramount to a successful time- series program and is contingent upon the use of appropriate sampling equipment that is well- maintained and operationally sound. A field sampling equipment maintenance program is administered by the marine technicians. The program consists of a documented inspection of field equipment at regular intervals. A written record of repairs, modifications and any other pertinent information is also maintained. In addition, diagrams outlining all sampling equipment and assembly procedures for sediment trap and in situ primary productivity arrays, radio direction finder tracking equipment, Argos satellite transmitter and on- deck incubation system have been prepared and are updated as necessary. Sample collection quality control measures are based upon the concept of applying time- tested oceanographic sampling techniques in a standardized and coordinated manner that is supervised or conducted by experienced personnel (details of each sampling procedure are outlined in the following chapters). Sampling metadata are recorded on log sheets at the time of collection, identifying the type of samples collected, cruise, station, time, cast number, sample number and any other pertinent information. These metadata, along with copies of the CTD console log and property vs. Records are maintained to identify sample tracking from collection through analysis and data reporting. Any problems associated with a particular sample are noted on the appropriate log sheet or data file and are evaluated relative to routine quality control procedures. Analytical Facilities. Most analyses are conducted at the University of Hawaii at Manoa in modern, well- equipped research laboratories. Since HOT- 4. 5 (Jan 1. DIC and alkalinity measurements have been conducted at the Mt. Soledad laboratories of the Scripps Institution of Oceanography. Specialized analytical equipment used in the JGOFS project include: Packard model #4. UIC model #5. 01. Biospherical microprocessor- controlled ATP photometer, Perkin- Elmer model #2. Europa ANCA- NT system carbon/nitrogen analyzers, Technicon autoanalyzer and accessories, automatic Winkler titration system consisting of Brinkmann Dosimat 6. Orion EA 9. 40 ion analyzer and IBM compatible computer, Guildline Autosal model 8. A salinometer, Antek model #7. |
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